Based on high-throughput qPCR (HT-qPCR) quantitative detection technology, it is used for the comprehensive analysis of the quantitative detection of genes involved in the microbial arsenic (As) cycle. The arsenic functional gene chip contains 80 pairs of primers, targeting 19 microbial-mediated functional genes for As(V) reduction, As(III) oxidation, As transport, As methylation and demethylation, and one 16S rRNA gene. Arsenic functional gene chips have the advantages of high throughput, low sample volume, low cost, short cycle, and simple operation, and can be widely used in various types of samples. Arsenic functional microarrays offer unprecedented potential for targeted and comprehensive quantification of As genes in environmental samples.