Based on the high-throughput qPCR (HT-qPCR) quantitative detection technology, the corresponding qPCR primers were designed for antibiotic resistance genes and packaged into thin-layer metal alloy nanopore chips to obtain high-throughput qPCR chips. At the same time, based on the SmartChip Real-Time PCR System, high-throughput automatic micro-sampling and gene quantification analysis platform, 5,184 reactions and data analysis can be completed at one time and at high speed, and the target gene detection and quantitative calculation with high efficiency, high throughput, high accuracy and high sensitivity can be carried out on multiple samples, avoiding the shortcomings of the traditional qPCR method such as single, high cost and low efficiency of genetic detection.