Metatranscriptome sequencing is the study of the entire microbial community in a specific environment, which gets rid of the technical limitations of microorganisms that are difficult to isolate and culture, and only needs to extract the total RNA of all microorganisms from the sample for sequencing.
Metatranscriptome sequencing uses the Novaseq second-generation high-throughput sequencing platform to comprehensively sequence the RNA of microbial communities in the samples, and through assembly, gene prediction, gene annotation and abundance calculation, the genetic diversity and molecular ecological information such as species composition, transcriptional regulation of functional genes and metabolic pathways in the samples are mined.
Features:
l Microbial communities can be identified at the transcriptional level and community characteristics can be studied;
l Identification of target pathogens;
l Excavation of live microorganisms and their functions;
l Focus on key genes and explore gene function and metabolic pathways.