Microbial resequencing refers to a sequencing analysis technology that performs high-throughput data analysis on a microorganism by comparing it with a close reference genome. Through resequencing, a series of variation information such as SNP, InDel, and SV of the target microbial genome from the reference genome can be obtained, and the trait differences between the genomes can be predicted and analyzed, so as to complete the study of the target microorganisms.