Traditional real-time PCR (qPCR) is commonly used for the detection of key genes and the validation of omics data because it can perform relative/absolute quantification of specific genes in a sample in real time, but traditional qPCR with low throughput, cumbersome operation and high experimental cost has been difficult to meet the increasingly complex needs of researchers. The high-throughput qPCR chip product just makes up for the shortcomings of traditional qPCR detection.

High-throughput qPCR (HT-qPCR) chip quantitative detection technology is to design the corresponding qPCR primers for the functional genes under study, and package them into thin-layer metal alloy nanopore chips to obtain high-throughput qPCR chips. At the same time, based on the SmartChip Real-Time PCR System, high-throughput automatic micro-sampling and gene quantification analysis platform, 5,184 reactions and data analysis can be completed at one time and at high speed, and the target gene detection and quantitative calculation with high efficiency, high throughput, high accuracy and high sensitivity can be carried out on multiple samples, avoiding the shortcomings of the traditional qPCR method such as single, high cost and low efficiency of genetic detection.

High-throughput qPCR chip detection platform