Bacterial completion map refers to the comprehensive use of second-generation or third-generation sequencing technology to carry out genome sequencing and assembly according to the specific information of the strain, and finally obtain a complete genome sequence without gap. The bacterial completion map can obtain comprehensive and rich genome information of bacteria, so as to dig deep into functional genes from multiple perspectives such as pathogenicity, drug resistance, and environmental adaptability, and conduct in-depth research on the characteristics of different phenotypes of microorganisms.